 Steve CarellGru (voice) | Gru (voice) |
 Kristen WiigLucy (voice) | Lucy (voice) |
 Will FerrellMaxime (voice) | Maxime (voice) |
 Sofía VergaraValentina (voice) | Valentina (voice) |
 Miranda CosgroveMargo (voice) | Margo (voice) |
 Dana Gaier EllisEdith (voice) | Edith (voice) |
 Madison PolanAgnes (voice) | Agnes (voice) |
 Pierre CoffinMinions (voice) | Minions (voice) |
 Chris RenaudPrincipal Übelschlecht (voice) | Principal Übelschlecht (voice) |
 Steve CooganSilas (voice) | Silas (voice) |
 Stephen ColbertPerry Prescott (voice) | Perry Prescott (voice) |
 Chloe FinemanPatsy Prescott (voice) | Patsy Prescott (voice) |
 Joey KingPoppy Prescott (voice) | Poppy Prescott (voice) |
 Laraine NewmanMelora / Additional Voices (voice) | Melora / Additional Voices (voice) |
 John DiMaggioBus Driver (voice) | Bus Driver (voice) |
 Tara StrongAdditional Voices (voice) | Additional Voices (voice) |
 Romesh RanganathanAdditional Voices (voice) | Additional Voices (voice) |
 Brad AblesonAdditional Voices (voice) | Additional Voices (voice) |
 Barbara HarrisAdditional Voices (voice) | Additional Voices (voice) |
| EEden BoultonAdditional Voices (voice) | Additional Voices (voice) |
 JP KarliakAdditional Voices (voice) | Additional Voices (voice) |
 Cathy CavadiniAdditional Voices (voice) | Additional Voices (voice) |
 Arif S. KinchenAdditional Voices (voice) | Additional Voices (voice) |
 Will CollyerAdditional Voices (voice) | Additional Voices (voice) |
 Jeremy MaxwellAdditional Voices (voice) | Additional Voices (voice) |
 Abby CradenAdditional Voices (voice) | Additional Voices (voice) |
 Ken DaurioAdditional Voices (voice) | Additional Voices (voice) |
 Khary PaytonAdditional Voices (voice) | Additional Voices (voice) |
 John DeMitaAdditional Voices (voice) | Additional Voices (voice) |
 Aaron ForsAdditional Voices (voice) | Additional Voices (voice) |
