 Steve CarellGru / Dru (voice) | Gru / Dru (voice) |
 Kristen WiigLucy Wilde (voice) | Lucy Wilde (voice) |
 Trey ParkerBalthazar Bratt (voice) | Balthazar Bratt (voice) |
 Miranda CosgroveMargo (voice) | Margo (voice) |
 Dana GaierEdith (voice) | Edith (voice) |
 Nhev ScharrelAgnes (voice) | Agnes (voice) |
 Pierre CoffinMel / Minions / Museum Director / Additional Voice (voice) | Mel / Minions / Museum Director / Additional Voice (voice) |
 Steve CooganFritz / Silas Ramsbottom (voice) | Fritz / Silas Ramsbottom (voice) |
 Julie AndrewsGru’s Mom (voice) | Gru’s Mom (voice) |
 Jenny SlateValerie Da Vinci (voice) | Valerie Da Vinci (voice) |
 Michael BeattieScar-Faced Man / TMZ Announcer (voice) | Scar-Faced Man / TMZ Announcer (voice) |
 Andy NymanClive the Robot (voice) | Clive the Robot (voice) |
 Adrian CiscatoNiko (voice) | Niko (voice) |
 Brian T. DelaneyCommercial Announcer / Military Officer / Additional Voice (voice) | Commercial Announcer / Military Officer / Additional Voice (voice) |
| KKatia SaponenkoNiko’s Mother (voice) | Niko’s Mother (voice) |
 Ken DaurioCelebrity (voice) | Celebrity (voice) |
| JJude AlpersCommercial Kid (voice) | Commercial Kid (voice) |
 Cory WallsVincenzo (voice) | Vincenzo (voice) |
| SSophie M. SiadatpourLittle Girl (voice) | Little Girl (voice) |
 Lori AlanAdditional Voice (voice) | Additional Voice (voice) |
 Carlos AlazraquiAdditional Voice (voice) | Additional Voice (voice) |
 Kyle BaldaAdditional Voice (voice) | Additional Voice (voice) |
 Bob BergenAdditional Voice (voice) | Additional Voice (voice) |
 John CyganAdditional Voice (voice) | Additional Voice (voice) |
 Bill FarmerAdditional Voice (voice) | Additional Voice (voice) |
 Danny MannAdditional Voice (voice) | Additional Voice (voice) |
| SStephanie De MeautisAdditional Voice (voice) | Additional Voice (voice) |
 Bruno DequierAdditional Voice (voice) | Additional Voice (voice) |
 Teresa GanzelAdditional Voice (voice) | Additional Voice (voice) |
 Jess HarnellAdditional Voice (voice) | Additional Voice (voice) |
