 Steve CarellGru (voice) | Gru (voice) |
 Kristen WiigLucy (voice) | Lucy (voice) |
 Benjamin BrattEduardo / El Macho (voice) | Eduardo / El Macho (voice) |
 Miranda CosgroveMargo (voice) | Margo (voice) |
 Dana GaierEdith (voice) | Edith (voice) |
 Elsie FisherAgnes (voice) | Agnes (voice) |
 Russell BrandDr. Nefario (voice) | Dr. Nefario (voice) |
 Ken JeongFloyd (voice) | Floyd (voice) |
 Steve CooganSilas (voice) | Silas (voice) |
 Moisés AriasAntonio (voice) | Antonio (voice) |
 Nasim PedradJillian (voice) | Jillian (voice) |
 Kristen SchaalShannon (voice) | Shannon (voice) |
 Pierre CoffinKevin / Bob / Stuart / Additional Minions / Evil Minions (voice) | Kevin / Bob / Stuart / Additional Minions / Evil Minions (voice) |
 Chris RenaudAdditional Minions / Evil Minions / Italian Waiter (voice) | Additional Minions / Evil Minions / Italian Waiter (voice) |
 Nickolai StoilovArctic Lab Guards (voice) | Arctic Lab Guards (voice) |
 Vanessa BayerFlight Attendant (voice) | Flight Attendant (voice) |
 Ava AcresAdditional Voices (voice) | Additional Voices (voice) |
 Lori AlanAdditional Voices (voice) | Additional Voices (voice) |
 Jack AngelAdditional Voices (voice) | Additional Voices (voice) |
 Eva BellaAdditional Voices (voice) | Additional Voices (voice) |
 Georgia CookAdditional Voices (voice) | Additional Voices (voice) |
 John CyganAdditional Voices (voice) | Additional Voices (voice) |
 Debi DerryberryAdditional Voices (voice) | Additional Voices (voice) |
 Jess HarnellAdditional Voices (voice) | Additional Voices (voice) |
 Danny MannAdditional Voices (voice) | Additional Voices (voice) |
 Mona MarshallAdditional Voices (voice) | Additional Voices (voice) |
 Mickie McGowanAdditional Voices (voice) | Additional Voices (voice) |
 Mason McNultyAdditional Voices (voice) | Additional Voices (voice) |
 Alec MedlockAdditional Voices (voice) | Additional Voices (voice) |
 Laraine NewmanAdditional Voices (voice) | Additional Voices (voice) |
