 Steve CarellGru (voice) | Gru (voice) |
 Jason SegelVector (voice) | Vector (voice) |
 Russell BrandDr. Nefario (voice) | Dr. Nefario (voice) |
 Julie AndrewsGru's Mother (voice) | Gru's Mother (voice) |
 Will ArnettMr. Perkins (voice) | Mr. Perkins (voice) |
 Kristen WiigMiss Hattie (voice) | Miss Hattie (voice) |
 Miranda CosgroveMargo (voice) | Margo (voice) |
 Dana GaierEdith (voice) | Edith (voice) |
 Elsie FisherAgnes (voice) | Agnes (voice) |
 Pierre CoffinTim / Bob / Mark / Phil / Stuart (voice) | Tim / Bob / Mark / Phil / Stuart (voice) |
 Chris RenaudDave (voice) | Dave (voice) |
 Jemaine ClementJerry (voice) | Jerry (voice) |
 Jack McBrayerCarnival Barker / Tourist Dad (voice) | Carnival Barker / Tourist Dad (voice) |
 Danny McBrideFred McDade (voice) | Fred McDade (voice) |
 Mindy KalingTourist Mom (voice) | Tourist Mom (voice) |
 Rob HuebelAnchorman (voice) | Anchorman (voice) |
 Ken DaurioEgyptian Guard (voice) | Egyptian Guard (voice) |
 Ken JeongTalk Show Host (voice) | Talk Show Host (voice) |
| CCharles BrightAdditional Voices (voice) | Additional Voices (voice) |
 Katie LeighAdditional Voices (voice) | Additional Voices (voice) |
 Ranjani BrowAdditional Voices (voice) | Additional Voices (voice) |
 Scott MenvilleAdditional Voices (voice) | Additional Voices (voice) |
 Holly DorffAdditional Voices (voice) | Additional Voices (voice) |
| EEdie MirmanAdditional Voices (voice) | Additional Voices (voice) |
 Jackie GonneauAdditional Voices (voice) | Additional Voices (voice) |
 Al RodrigoAdditional Voices (voice) | Additional Voices (voice) |
 Wendy HoffmannAdditional Voices (voice) | Additional Voices (voice) |
| JJakob RostonAdditional Voices (voice) | Additional Voices (voice) |
 James KysonAdditional Voices (voice) | Additional Voices (voice) |
 John Hans TesterAdditional Voices (voice) | Additional Voices (voice) |
