 Mae WhitmanBarbara Gordon/Batgirl (voice) | Barbara Gordon/Batgirl (voice) |
| AAnais FairweatherSupergirl / Kara Zor-El (voice) | Supergirl / Kara Zor-El (voice) |
 Grey DeLisleWonder Woman / Platinum (voice) | Wonder Woman / Platinum (voice) |
 Teala DunnBumblebee / Karen Beecher / Artemiz (voice) | Bumblebee / Karen Beecher / Artemiz (voice) |
 Tara StrongHarley Quinn/ Harleen Quinzel / Poison Ivy / Pamela Isley (voice) | Harley Quinn/ Harleen Quinzel / Poison Ivy / Pamela Isley (voice) |
 Phil LaMarrDoc Magnus (voice) | Doc Magnus (voice) |
 John DiMaggioAmbassador Bek (voice) | Ambassador Bek (voice) |
 Jessica DiCiccoStar Sapphire / Carol Ferris / Lashina (voice) | Star Sapphire / Carol Ferris / Lashina (voice) |
 Yvette Nicole BrownPrincipal Waller (voice) | Principal Waller (voice) |
 Nika FuttermanHawkgirl (voice) | Hawkgirl (voice) |
 Tania GunadiLady Shiva (voice) | Lady Shiva (voice) |
 Tom KennySinestro / Lobo (voice) | Sinestro / Lobo (voice) |
 Danica McKellarFrost (voice) | Frost (voice) |
 Fred TatascioreBrainia / Kryptomites (voice) | Brainia / Kryptomites (voice) |
 Hynden WalchStarfire / Koriand'r/ Blackfire (voice) | Starfire / Koriand'r/ Blackfire (voice) |
 Alexis G. ZallLois Lane (voice) | Lois Lane (voice) |
