 Ed AsnerJ. Jonah Jameson (voice) | J. Jonah Jameson (voice) |
 Dorian HarewoodLonnie / Tombstone (voice) | Lonnie / Tombstone (voice) |
 Christopher Daniel BarnesSpider-Man / Peter Parker (voice) | Spider-Man / Peter Parker (voice) |
 Sara BallantineMary Jane Watson (voice) | Mary Jane Watson (voice) |
 Leigh-Allyn BakerAlisha Silvermane (voice) | Alisha Silvermane (voice) |
 Maxwell CaulfieldAlistair Smythe (voice) | Alistair Smythe (voice) |
 Linda GaryAunt May (voice) | Aunt May (voice) |
 Gary ImhoffHarry Osborn (voice) | Harry Osborn (voice) |
 Roscoe Lee BrowneKingpin (voice) | Kingpin (voice) |
 Rodney SaulsberryJoe 'Robbie' Robertson (voice) | Joe 'Robbie' Robertson (voice) |
 Majel BarrettAnna Watson (voice) | Anna Watson (voice) |
 Edward AlbertMatt Murdock / Daredevil (voice) | Matt Murdock / Daredevil (voice) |
 Nick JamesonRichard Fisk (voice) | Richard Fisk (voice) |
 Amy HillSusan Choi (voice) | Susan Choi (voice) |
 Dawnn LewisDetective Terri Lee (voice) | Detective Terri Lee (voice) |
 Joan LeeMadame Web (voice) | Madame Web (voice) |
 David WarnerLandon (voice) | Landon (voice) |
 Neil RossNorman Osborn (voice) | Norman Osborn (voice) |
 Alfonso RibeiroRandy Robertson (voice) | Randy Robertson (voice) |
 Iona MorrisMartha Robertson (voice) | Martha Robertson (voice) |
