| JJean Claude RicquebourgChristopher Columbus | Christopher Columbus |
| JJosé Antonio LorenteSelf - Geneticist / Narrator (voice) | Self - Geneticist / Narrator (voice) |
 Cristóbal Colón de CarvajalSelf - Descendant / Narrator (voice) | Self - Descendant / Narrator (voice) |
| MMarcial CastroSelf - Historian | Self - Historian |
| EEsther MartínSelf - Journalist | Self - Journalist |
| AAinhoa del CastilloSelf - Colón ADN Coordinator | Self - Colón ADN Coordinator |
| RRegis Francisco LópezSelf - Journalist | Self - Journalist |
| CCarlos BayarriSelf - Museo Pickman Curator | Self - Museo Pickman Curator |
| JJuan Manuel MartínSelf - Edaphology Professor | Self - Edaphology Professor |
| RRafael DelgadoSelf - Edaphology Professor | Self - Edaphology Professor |
| VVanessa GómezSelf - Journalist | Self - Journalist |
| AAlfonso SanzSelf - Castilian Theory Promoter | Self - Castilian Theory Promoter |
| FFernando BrancoSelf - 1st Portuguese Theory Promoter | Self - 1st Portuguese Theory Promoter |
| JJosé Matos SilvaSelf - 2nd Portuguese Theory Promoter | Self - 2nd Portuguese Theory Promoter |
| EEduardo EstebanSelf - Galician Theory Promoter | Self - Galician Theory Promoter |
| IInmaculada AlemánSelf - Forensic Anthropologist | Self - Forensic Anthropologist |
| JJosé AguiñoSelf - Restorer | Self - Restorer |
| GGabriel MartorellSelf - Balearic Theory Promoter | Self - Balearic Theory Promoter |
| JJosé María ErcillaSelf - Agote Theory Promoter | Self - Agote Theory Promoter |
| FFrancesc AlbardanerSelf - Jewish Theory Promoter | Self - Jewish Theory Promoter |
| CCristina Gutiérrez GonzálezQueen Isabel | Queen Isabel |
| ÁÁngel González ÁlvarezKing Fernando | King Fernando |
| JJuan Frías SanzPrince of Viana | Prince of Viana |
| SSantiago Salgado BellidoDiego Colón - Son | Diego Colón - Son |
| SSaúl de Lucas MuñozHernando Colón | Hernando Colón |
| PPepe PereiraDiego Colón - Brother | Diego Colón - Brother |
| CCristina Vargas CarracedoMargalida Colom | Margalida Colom |
| NNerea García MartínFilipa Moniz | Filipa Moniz |
| JJavier García BotelloYoung Columbus | Young Columbus |
| AAdrián Casado CuadradoChild Columbus (Medina) | Child Columbus (Medina) |
