 Fredric MarchChristopher Columbus | Christopher Columbus |
 Florence EldridgeQueen Isabella | Queen Isabella |
 Francis L. SullivanFrancisco de Bobadilla | Francisco de Bobadilla |
 Kathleen RyanBeatriz Enriquez de Arana | Beatriz Enriquez de Arana |
 Derek BondDiego de Arana | Diego de Arana |
 Nora SwinburneJoanna de Torres | Joanna de Torres |
 Abraham SofaerLuis de Santangel | Luis de Santangel |
 Linden TraversBeatriz de Peraza | Beatriz de Peraza |
 James Robertson JusticeMartin Alonso Pinzon | Martin Alonso Pinzon |
 Dennis VanceFrancisco Pinzon | Francisco Pinzon |
 Richard AherneVicente Yañez Pinzon | Vicente Yañez Pinzon |
 Felix AylmerFather Perez | Father Perez |
 Francis ListerKing Ferdinand | King Ferdinand |
 Edward RigbyPedro | Pedro |
 Niall MacGinnisJuan de la Cosa | Juan de la Cosa |
 Ralph TrumanCaptain | Captain |
 Ronald AdamTalavera | Talavera |
| GGuy Le FeuvreAdmiral | Admiral |
| LLyn EvansLope | Lope |
| DDavid ColeDiego Columbus - Columbus' Son | Diego Columbus - Columbus' Son |
 Hugh PryseAlmoner | Almoner |
| SStuart LindsellPrior | Prior |
 Valentine DyallNarrator (voice) | Narrator (voice) |
| TThelma GriggLady in waiting (uncredited) | Lady in waiting (uncredited) |
 Anthony SteelMessenger (uncredited) | Messenger (uncredited) |
