 Larry the Cable GuyMater (voice) | Mater (voice) |
 Owen WilsonLightning McQueen (voice) | Lightning McQueen (voice) |
 Michael CaineFinn McMissile (voice) | Finn McMissile (voice) |
 Emily MortimerHolley Shiftwell (voice) | Holley Shiftwell (voice) |
 Suzy Eddie IzzardSir Miles Axlerod (voice) | Sir Miles Axlerod (voice) |
 John TurturroFrancesco Bernoulli (voice) | Francesco Bernoulli (voice) |
 Brent MusburgerBrent Mustangburger (voice) | Brent Mustangburger (voice) |
 Joe MantegnaGrem (voice) | Grem (voice) |
 Thomas KretschmannProfessor Z (voice) | Professor Z (voice) |
 Peter JacobsonAcer (voice) | Acer (voice) |
 Bonnie HuntSally (voice) | Sally (voice) |
 Darrell WaltripDarrell Cartrip (voice) | Darrell Cartrip (voice) |
 Franco NeroUncle Topolino (voice) | Uncle Topolino (voice) |
 David HobbsDavid Hobbscap (voice) | David Hobbscap (voice) |
| PPatrick WalkerMel Dorado (voice) | Mel Dorado (voice) |
 Tony ShalhoubLuigi (voice) | Luigi (voice) |
 Jeff GarlinOtis (voice) | Otis (voice) |
 Michel MichelisTomber (voice) | Tomber (voice) |
 Jason IsaacsSiddeley / Leland Turbo (voice) | Siddeley / Leland Turbo (voice) |
 Lloyd SherrFillmore / Combat Ship (voice) | Fillmore / Combat Ship (voice) |
 Bruce CampbellRod 'Torque' Redline (voice) | Rod 'Torque' Redline (voice) |
 Teresa GallagherMater's Computer (voice) | Mater's Computer (voice) |
 Jenifer LewisFlo (voice) | Flo (voice) |
 Stanley TownsendVictor Hugo / Vladimir Trunkov / Ivan the Tow Truck (voice) | Victor Hugo / Vladimir Trunkov / Ivan the Tow Truck (voice) |
 Velibor TopicAlexander Hugo (voice) | Alexander Hugo (voice) |
 Sig HansenCrabby (voice) | Crabby (voice) |
 Guido QuaroniGuido (voice) | Guido (voice) |
 Vanessa RedgraveThe Queen / Mama Topolino (voice) | The Queen / Mama Topolino (voice) |
 John MainieriJ. Curby Gremlin (voice) | J. Curby Gremlin (voice) |
 Bradford LewisTubbs Pacer (voice) | Tubbs Pacer (voice) |
