Joe embarks on a personal vendetta to end Duke's life, while the conniving Usagi launches her own crusade against the most beautiful unicorn in the world. Can Duke defend against both enemies?
| Actor | Role |
|---|---|
 Autumn IvyUsagi | Usagi |
| SSteve RimpiciDuke (voice) | Duke (voice) |
 Steve RudzinskiJoe | Joe |
| BB. BarnabeiRobbie | Robbie |
| PPaul BilboBen | Ben |
 Melissa HeflinDetective Duck | Detective Duck |
| BBilly PeckRadical Rat | Radical Rat |
| SScott LewisLenny | Lenny |
| JJudy H.R. KirbyJudy | Judy |
 Jessa Jupiter FluxSerena | Serena |
| IIan SunnyPeter | Peter |
| BBrenan MarshThe Lionheart | The Lionheart |
| LLyzz MayLiz S. Kennedy | Liz S. Kennedy |
| JJon St. JohnFuture Robbie | Future Robbie |
 Aleen IsleyFake Shemp | Fake Shemp |
| EEric J HolmesCreep | Creep |
| MMarci LeighJen | Jen |
| AAmanda DavisDana Denovchek | Dana Denovchek |
| SSteven HenryBilly Beads | Billy Beads |
| MMatt MahonThe Mattster | The Mattster |
| MMichael WolfeThe Door Wolf | The Door Wolf |
| CCody RuchSmoking Man 2 | Smoking Man 2 |
| DDavid HubbardRichard | Richard |
| PPreston EverestPurple Fighter | Purple Fighter |
| DDerek DillingerYellow Fighter | Yellow Fighter |
| KKatie ArquetteBlue Fighter | Blue Fighter |
 John CathelinePink Fighter | Pink Fighter |
| BBrian HaasPsycho Ape Fan | Psycho Ape Fan |
| MMichael SmythShingles Fan | Shingles Fan |