The story follows three men—C.O. Darlington, C.O. Drudge, and C.O. Dusenberry (whose shared initials give the film its title)—who deceive their wives by faking illnesses to go on a secret vacation together.
| Actor | Role |
|---|---|
 Harry DavenportC.O. Darlington | C.O. Darlington |
 Hughie MackC.O. Drudge | C.O. Drudge |
 Eulalie JensenMrs. Darlington | Mrs. Darlington |
 Edwina RobbinsMrs. Dusenberry | Mrs. Dusenberry |
 William R. DunnWallie Walton | Wallie Walton |
 William SheaFarmer Jones | Farmer Jones |
 Ethel CorcoranIwilla Jones | Iwilla Jones |
 Mary AndersonIrva Jones | Irva Jones |
| CCharles BrownC.O. Dusenberry | C.O. Dusenberry |
| MMabel KellyMrs. Drudge | Mrs. Drudge |
| JJack BulgerWillie Willing | Willie Willing |
| SStephen LennonWilton Wallford | Wilton Wallford |
| MMinnie StoreyMrs. Jones | Mrs. Jones |
| RRuth EdwardsIrma Jones | Irma Jones |
| CCharles Michael EdwardsLem | Lem |