The tiny independent duchy of Ferrara is located between Casare Borgia's Rome and Venice, and Borgia has plans to conquer Venice via Ferrara. He murders his sister's husband and makes it appear that Alfonso D'Este of Ferrara was behind the killing. To avenge herself against Ferrara and D'Este, Lucretia Borgia marries D'Este and intends to poison him. But...she falls in love with him.
| Actor | Role |
|---|---|
 Paulette GoddardLucretia Borgia | Lucretia Borgia |
 John LundAlfonso D'Este | Alfonso D'Este |
 Macdonald CareyCesare Borgia | Cesare Borgia |
 Albert DekkerVanetti | Vanetti |
 John SuttonPrince Bisceglie | Prince Bisceglie |
 Raymond BurrMichelotto | Michelotto |
 Donald RandolphTiziano | Tiziano |
| CCharles DaytonBastino | Bastino |
 Anthony CarusoCaptain of the Guard | Captain of the Guard |
| DDick FooteNegligent Sentry | Negligent Sentry |
 William FarnumConti Peruzzi | Conti Peruzzi |
 Kate Drain LawsonGemma | Gemma |
 Nicholas JoyChamberlain | Chamberlain |
 Fritz LeiberFilippo | Filippo |
 Rose HobartLady Eleanora | Lady Eleanora |
 Douglas SpencerFalse Physician | False Physician |
 Nestor PaivaMayor | Mayor |
 Frank PugliaBolfi | Bolfi |
 Houseley StevensonCouncillor | Councillor |
 Robert GreigCouncillor | Councillor |
 Don BeddoeCouncillor | Councillor |
 Ian WolfeCouncillor (uncredited) | Councillor (uncredited) |