The brutal hunt for the pure bloods continues as the ultimate vampire hunter, Blade, battles to defeat the House of Chthon, an ancient evil sect of vampires that is growing stronger everyday. Led by the vampire overlord Marcus, their objective is to create a vaccine that will give rise to a new breed of super vampires, immune to vampire weaknesses.
| Actor | Role |
|---|---|
 Sticky FingazBlade | Blade |
 Jill WagnerKrista Starr | Krista Starr |
 Nelson LeeShen | Shen |
 Neil JacksonMarcus Van Sciver | Marcus Van Sciver |
 Jessica GowerChase | Chase |
 Bill MondyDetective Boone | Detective Boone |
 David PalffyFritz | Fritz |
 David KoppZack | Zack |
 P. Lynn JohnsonLisa | Lisa |
 Randy QuaidMelvin | Melvin |