 Margot RobbieHarley Quinn | Harley Quinn |
 Rosie PerezRenee Montoya | Renee Montoya |
 Mary Elizabeth WinsteadHelena Bertinelli / Huntress | Helena Bertinelli / Huntress |
 Jurnee Smollett-BellDinah Lance / Black Canary | Dinah Lance / Black Canary |
 Ewan McGregorRoman Sionis | Roman Sionis |
 Ella Jay BascoCassandra Cain | Cassandra Cain |
 Chris MessinaVictor Zsasz | Victor Zsasz |
 Ali WongEllen Yee | Ellen Yee |
 David UrySleazy Breeder | Sleazy Breeder |
 Sara MontezShallow Friend #1 / Kathrine | Shallow Friend #1 / Kathrine |
 Izabel PakzadShallow Friend #2 | Shallow Friend #2 |
 Daniel BernhardtSionis' Chauffeur | Sionis' Chauffeur |
 Kc StrubbeNaïve Teammate | Naïve Teammate |
 Jacky ShuSkeptical Teammate | Skeptical Teammate |
 Paloma Esparza RabinovBitchy Teammate | Bitchy Teammate |
 Derek WilsonDetective Tim Munroe | Detective Tim Munroe |
 Joe Bucaro IIICarlos Rossi | Carlos Rossi |
| JJames Henry Williams Jr.Ace Chemical Police Officer | Ace Chemical Police Officer |
 François ChauMr. Keo | Mr. Keo |
| MMiyuki MatsunagaMrs. Keo | Mrs. Keo |
 Anna MikamiMiss Keo (16 yrs) | Miss Keo (16 yrs) |
| BBruno OliverBodega Cook (Sal) | Bodega Cook (Sal) |
 Keisha TuckerRoller Derby Girl | Roller Derby Girl |
 Matthew WilligHappy | Happy |
 Jeremy DenzlingerDesk Sergeant | Desk Sergeant |
 Michael MasiniOfficer Drago | Officer Drago |
 Charles CarpenterProcessing Officer | Processing Officer |
 Daniel ColePatrol Cop | Patrol Cop |
 Jesse BushPatrol Cop | Patrol Cop |
 Steven WilliamsCaptain Patrick Erickson | Captain Patrick Erickson |
