A girl, abandoned by her mother when she was three, moves to a small town in Florida with her father. There, she adopts an orphaned dog she names Winn-Dixie. The bond between the girl and her special companion brings together the people in a small Florida town and heals her own troubled relationship with her father.
| Actor | Role |
|---|---|
 AnnaSophia RobbOpal | Opal |
 Jeff DanielsPreacher | Preacher |
 Cicely TysonGloria Dump | Gloria Dump |
 Eva Marie SaintMiss Franny | Miss Franny |
 Dave MatthewsOtis | Otis |
 Courtney JinesAmanda Wilkenson | Amanda Wilkenson |
 Elle FanningSweety Pie Thomas | Sweety Pie Thomas |
 Luke BenwardStevie Dewberry | Stevie Dewberry |
 Nick PriceDunlap Dewberry | Dunlap Dewberry |
| BB.J. HopperMr. Alfred | Mr. Alfred |
| MMarca PriceMrs. Dewberry | Mrs. Dewberry |
 Lenore BanksMrs. Detweller | Mrs. Detweller |
 John McConnellStore Manager | Store Manager |
 Harland WilliamsPoliceman | Policeman |
| WWilliam Arthur PittsChurchgoer 1 | Churchgoer 1 |
| EEnid TrotinerChurchgoer 2 | Churchgoer 2 |
| CCharles A. DaigleChurchgoer 3 | Churchgoer 3 |
| CClarice H. GauthreauxChurchgoer 4 | Churchgoer 4 |
| GGuy G. GauthreauxChurchgoer 5 | Churchgoer 5 |
 Jared DayMike | Mike |
| SSergio KatoSPCA Officer (uncredited) | SPCA Officer (uncredited) |
| MMatthew D. MillerChurch Member (uncredited) | Church Member (uncredited) |
| RRoss RouillierShopper (uncredited) | Shopper (uncredited) |