The Joker is aided in his Halloween quest to render modern technology useless and take over Gotham City by Solomon Grundy, Silver Banshee, Clayface and Scarecrow.
| Actor | Role |
|---|---|
 Roger Craig SmithBatman (voice) | Batman (voice) |
 Troy BakerThe Joker (voice) | The Joker (voice) |
 Chris DiamantopoulosGreen Arrow (voice) | Green Arrow (voice) |
 Kari WührerSilver Banshee (voice) | Silver Banshee (voice) |
 Noel FisherGogo Shoto (voice) | Gogo Shoto (voice) |
 Khary PaytonCyborg (voice) | Cyborg (voice) |
 Will FriedleNightwing (voice) | Nightwing (voice) |
 Yuri LowenthalRed Robin (voice) | Red Robin (voice) |
 Fred TatascioreSolomon Grundy (voice) | Solomon Grundy (voice) |
 Brian T. DelaneyScarecrow (voice) | Scarecrow (voice) |
 Dave B. MitchellClayface (voice) | Clayface (voice) |
 Richard EpcarCommissioner Gordon (voice) | Commissioner Gordon (voice) |
 Eric BauzaHouston Raines (voice) | Houston Raines (voice) |
 Alastair DuncanAlfred (voice) | Alfred (voice) |
 Amanda TroopGladys Windsmere / Newscaster (voice) | Gladys Windsmere / Newscaster (voice) |
 Steve BlumGruff Cop / Watchman #2 (voice) | Gruff Cop / Watchman #2 (voice) |
 Cedric YarbroughDr. Silas Stone (voice) | Dr. Silas Stone (voice) |
| JJanell CoxAnna / Computer (voice) | Anna / Computer (voice) |