 Bruce GreenwoodBatman / Bruce Wayne (voice) | Batman / Bruce Wayne (voice) |
 Jensen AcklesRed Hood / Jason Todd (voice) | Red Hood / Jason Todd (voice) |
 Neil Patrick HarrisNightwing (voice) | Nightwing (voice) |
 Jason IsaacsRa's al Ghul (voice) | Ra's al Ghul (voice) |
 John DiMaggioJoker (voice) | Joker (voice) |
 Wade WilliamsBlack Mask (voice) | Black Mask (voice) |
 Carlos AlazraquiChi Chi / Thug #1 / Baton (voice) | Chi Chi / Thug #1 / Baton (voice) |
 Robert ClotworthyLeon / Thug (voice) | Leon / Thug (voice) |
 Gary ColeBobo / Commissioner Gordon / Shot / Guard (voice) | Bobo / Commissioner Gordon / Shot / Guard (voice) |
 Brian GeorgeRa's Assistant (voice) | Ra's Assistant (voice) |
 Kelly HuMs. Li (voice) | Ms. Li (voice) |
 Phil LaMarrRick / Bulk (voice) | Rick / Bulk (voice) |
| AAlexander MartellaYoung Robin / Jason Todd (voice) | Young Robin / Jason Todd (voice) |
 Vincent MartellaRobin / Jason Todd (voice) | Robin / Jason Todd (voice) |
 Jim PiddockAlfred (voice) | Alfred (voice) |
 Kevin Michael RichardsonTyler Bramford (voice) | Tyler Bramford (voice) |
 Andrea RomanoReporter #1 (voice) | Reporter #1 (voice) |
 Dwight SchultzFreddie / Drug Dealer (voice) | Freddie / Drug Dealer (voice) |
 Fred TatascioreMercenary #1 / Amazo / Guard (voice) | Mercenary #1 / Amazo / Guard (voice) |
 Keri TombazianReporter #2 (voice) | Reporter #2 (voice) |
 Bruce TimmThe Riddler (voice) | The Riddler (voice) |
 Michael VillaniReporter #3 (voice) | Reporter #3 (voice) |
