 Jensen AcklesBatman / Bruce Wayne (voice) | Batman / Bruce Wayne (voice) |
 Josh DuhamelHarvey Dent / Two-Face (voice) | Harvey Dent / Two-Face (voice) |
 Naya RiveraCatwoman / Selina Kyle (voice) | Catwoman / Selina Kyle (voice) |
 Billy BurkeCommissioner Gordon (voice) | Commissioner Gordon (voice) |
 Titus WelliverCarmine Falcone / Luigi Maroni (voice) | Carmine Falcone / Luigi Maroni (voice) |
 Troy BakerJoker (voice) | Joker (voice) |
 Laila BerzinsSofia Falcone (voice) | Sofia Falcone (voice) |
 Zach CallisonYoung Bruce Wayne (voice) | Young Bruce Wayne (voice) |
 David DastmalchianCalendar Man / Penguin (voice) | Calendar Man / Penguin (voice) |
 Alyssa DiazRenee Montoya (voice) | Renee Montoya (voice) |
 John DiMaggioMad Hatter (voice) | Mad Hatter (voice) |
 Robin Atkin DownesScarecrow / Thomas Wayne (voice) | Scarecrow / Thomas Wayne (voice) |
 Alastair DuncanAlfred (voice) | Alfred (voice) |
 Gary LeRoi GrayPearce (voice) | Pearce (voice) |
 Amy LandeckerBarbara Gordon / Carla Viti (voice) | Barbara Gordon / Carla Viti (voice) |
 Julie NathansonGilda (voice) | Gilda (voice) |
 Jim PirriSal Maroni (voice) | Sal Maroni (voice) |
 Katee SackhoffPoison Ivy (voice) | Poison Ivy (voice) |
 Fred TatascioreSolomon Grundy / Vincent Falcone (voice) | Solomon Grundy / Vincent Falcone (voice) |
 Rick D. WassermanBodyguard (voice) | Bodyguard (voice) |
