Following a brutal series of murders taking place on Halloween, Thanksgiving, and Christmas, Gotham City's young vigilante known as the Batman sets out to pursue the mysterious serial killer alongside police officer James Gordon and district attorney Harvey Dent.
| Actor | Role |
|---|---|
 Jensen AcklesBatman / Bruce Wayne (voice) | Batman / Bruce Wayne (voice) |
 Josh DuhamelHarvey Dent (voice) | Harvey Dent (voice) |
 Naya RiveraCatwoman / Selina Kyle (voice) | Catwoman / Selina Kyle (voice) |
 Billy BurkeCommissioner Gordon (voice) | Commissioner Gordon (voice) |
 Jack QuaidAlberto (voice) | Alberto (voice) |
 Titus WelliverCarmine Falcone (voice) | Carmine Falcone (voice) |
 Troy BakerJoker (voice) | Joker (voice) |
 Frances CallierNurse Tamara (voice) | Nurse Tamara (voice) |
 Greg ChunMickey Chen (voice) | Mickey Chen (voice) |
 David DastmalchianCalendar Man (voice) | Calendar Man (voice) |
 Alastair DuncanAlfred (voice) | Alfred (voice) |
 Gary LeRoi GrayPearce (voice) | Pearce (voice) |
 Amy LandeckerBarbara Gordon (voice) | Barbara Gordon (voice) |
 Julie NathansonGilda (voice) | Gilda (voice) |
 Jim PirriSal Maroni (voice) | Sal Maroni (voice) |
 Fred TatascioreSolomon Grundy (voice) | Solomon Grundy (voice) |