Atrocious serial killings on holidays in Gotham City send The World's Greatest Detective into action - confronting both organized crime and a unified front of classic DC Super-Villains - while attempting to stop the mysterious murderer.
| Actor | Role |
|---|---|
 Jensen AcklesBruce Wayne / Batman (voice) | Bruce Wayne / Batman (voice) |
 Josh DuhamelHarvey Dent (voice) | Harvey Dent (voice) |
 Naya RiveraSelina Kyle / Catwoman (voice) | Selina Kyle / Catwoman (voice) |
 Billy BurkeCommissioner James Gordon (voice) | Commissioner James Gordon (voice) |
 Titus WelliverCarmine 'The Roman' Falcone (voice) | Carmine 'The Roman' Falcone (voice) |
 Troy BakerJoker (voice) | Joker (voice) |
 David DastmalchianJulian Day / Calendar Man (voice) | Julian Day / Calendar Man (voice) |
 Julie NathansonGilda Dent (voice) | Gilda Dent (voice) |
 Alastair DuncanAlfred Pennyworth (voice) | Alfred Pennyworth (voice) |
 Jack QuaidAlberto Falcone (voice) | Alberto Falcone (voice) |
 Fred TatascioreSolomon Grundy (voice) | Solomon Grundy (voice) |
 Greg ChunMickey Chen (voice) | Mickey Chen (voice) |
 Gary LeRoi GrayPearce (voice) | Pearce (voice) |
 Jim PirriSal Maroni (voice) | Sal Maroni (voice) |
 Katee SackhoffPoison Ivy (voice) | Poison Ivy (voice) |