 Jason O'MaraBatman / Bruce Wayne (voice) | Batman / Bruce Wayne (voice) |
 Jennifer MorrisonCatwoman / Selina Kyle (voice) | Catwoman / Selina Kyle (voice) |
 Maury SterlingThomas Elliot (voice) | Thomas Elliot (voice) |
 Sean MaherNightwing / Dick Grayson (voice) | Nightwing / Dick Grayson (voice) |
 Geoffrey ArendThe Riddler / Edward Nigma (voice) | The Riddler / Edward Nigma (voice) |
 James GarrettAlfred Pennyworth (voice) | Alfred Pennyworth (voice) |
 Jerry O'ConnellSuperman / Kal-El / Clark Kent (voice) | Superman / Kal-El / Clark Kent (voice) |
 Rebecca RomijnLois Lane (voice) | Lois Lane (voice) |
 Bruce ThomasJim Gordon (voice) | Jim Gordon (voice) |
 Rainn WilsonLex Luthor (voice) | Lex Luthor (voice) |
 Peyton ListBatgirl / Barbara Gordon (voice) | Batgirl / Barbara Gordon (voice) |
 Stuart AllanRobin / Damian Wayne (voice) | Robin / Damian Wayne (voice) |
 Vanessa WilliamsAmanda Waller (voice) | Amanda Waller (voice) |
 Peyton ListPoison Ivy / Pamela Isley (voice) | Poison Ivy / Pamela Isley (voice) |
 Jason SpisakThe Joker (voice) | The Joker (voice) |
 Hynden WalchHarley Quinn (voice) | Harley Quinn (voice) |
 Adam GiffordBane / Clayface (voice) | Bane / Clayface (voice) |
 Sachie AlessioLady Shiva (voice) | Lady Shiva (voice) |
 Chris CoxScarecrow (voice) | Scarecrow (voice) |
 Tara StrongReporter (voice) | Reporter (voice) |
