 Bruce GreenwoodBruce Wayne / Batman (voice) | Bruce Wayne / Batman (voice) |
 Jennifer CarpenterSelina Kyle (voice) | Selina Kyle (voice) |
 Anthony Stewart HeadAlfred (voice) | Alfred (voice) |
 Scott PattersonJames Gordon (voice) | James Gordon (voice) |
 John DiMaggioChief Bullock / Big Bill Dust / Additional Voices (voice) | Chief Bullock / Big Bill Dust / Additional Voices (voice) |
 Yuri LowenthalHarvey Dent (voice) | Harvey Dent (voice) |
 Grey DeLisleSister Leslie / Jason / Additional Voices (voice) | Sister Leslie / Jason / Additional Voices (voice) |
 William SalyersHugo Strange (voice) | Hugo Strange (voice) |
 Bob JolesMayor Tolliver (voice) | Mayor Tolliver (voice) |
 Tara StrongMarlene (voice) | Marlene (voice) |
 Kari WührerBarbara Gordon / Pamela Isley (voice) | Barbara Gordon / Pamela Isley (voice) |
 Lincoln MelcherDickie (voice) | Dickie (voice) |
 Bruce TimmArkham Radio Man (voice) | Arkham Radio Man (voice) |
 David ForsethCyrus Gold (voice) | Cyrus Gold (voice) |
 Chris CoxFather Callahan / Additional Voices (voice) | Father Callahan / Additional Voices (voice) |
