 Will FriedleTerry McGinnis / Batman (voice) | Terry McGinnis / Batman (voice) |
 Kevin ConroyBruce Wayne (voice) | Bruce Wayne (voice) |
 Mark HamillThe Joker (voice) | The Joker (voice) |
 Angie HarmonCommissioner Barbara Gordon (voice) | Commissioner Barbara Gordon (voice) |
 Dean StockwellTim Drake (voice) | Tim Drake (voice) |
 Arleen SorkinHarley Quinn (voice) | Harley Quinn (voice) |
 Tara StrongBatgirl (voice) | Batgirl (voice) |
| MMathew ValenciaRobin (voice) | Robin (voice) |
 Andrea RomanoLaughing Boy (voice) | Laughing Boy (voice) |
 Melissa Joan HartDee Dee (voice) | Dee Dee (voice) |
 Henry RollinsBonk (voice) | Bonk (voice) |
 Michael RosenbaumGhoul (voice) | Ghoul (voice) |
 Don HarveyChucko (voice) | Chucko (voice) |
 Frank WelkerWoof / Ace (voice) | Woof / Ace (voice) |
 Rachael Leigh CookChelsea Cunningham (voice) | Chelsea Cunningham (voice) |
 Lauren TomDana Tan (voice) | Dana Tan (voice) |
 Teri GarrMary McGinnis (voice) | Mary McGinnis (voice) |
| RRyan O'DonohueMatt McGinnis (voice) | Matt McGinnis (voice) |
 Vernee WatsonMs. Carr (voice) | Ms. Carr (voice) |
 Mary ScheerMrs. Tim Drake (voice) | Mrs. Tim Drake (voice) |
 Bruce TimmGuard (voice) | Guard (voice) |
 Mark Jonathan DavisV.R. Newsman (voice) | V.R. Newsman (voice) |
