Batman battles his archenemy, the Joker, who instigates an elaborate plot to seize control of Arkham Asylum, trap Batman inside with many of his incarcerated foes, and threaten Gotham City with hidden bombs.
| Actor | Role |
|---|---|
 Kevin ConroyBatman / Bruce Wayne / Thomas Wayne | Batman / Bruce Wayne / Thomas Wayne |
 Mark HamillJoker / Scarface | Joker / Scarface |
 Arleen SorkinHarley Quinn | Harley Quinn |
 Tom KaneJim Gordon / Quincy Sharp / Amadeus Arkham | Jim Gordon / Quincy Sharp / Amadeus Arkham |
 Steve BlumKiller Croc | Killer Croc |
 Danny JacobsZsasz / Frank Boles | Zsasz / Frank Boles |
 Dino AndradeScarecrow | Scarecrow |
 Fred TatascioreBane | Bane |
 Wally WingertRiddler | Riddler |
 Kimberly BrooksOracle / Bruce Wayne (Child) | Oracle / Bruce Wayne (Child) |
 Cree SummerPenny Young | Penny Young |
 Adrienne BarbeauVoice of Arkham | Voice of Arkham |
 Chris CoxEddie Burlow | Eddie Burlow |
 Keith FergusonTV Voice | TV Voice |
| CChris GardnerHenry Smith / Zach Franklin | Henry Smith / Zach Franklin |
 Roger RoseWilliam North | William North |
 Duane R. Shepard Sr.Aaron Cash | Aaron Cash |
 Rick D. WassermanGotham Cop | Gotham Cop |
 James HoranJack Ryder | Jack Ryder |
 Kari WahlgrenVarious | Various |