Barbie and her best friends Teresa and Renee transform from hard-working gymnasts to undercover secret agents. When their amazing gymnastics skills catch the eye of a top-secret spy agency, the girls are soon following clues to a gem-stealing cat burglar, using high-tech gadgets, glam disguises and cute robo-pets to save the day.
| Actor | Role |
|---|---|
 Erica LindbeckBarbie (voice) | Barbie (voice) |
 Stephanie ShehRenee (voice) | Renee (voice) |
 Jenny PellicerTeresa (voice) | Teresa (voice) |
| AAlyssya SwalesChelsea (voice) | Chelsea (voice) |
| EElizabeth IrvingMila (voice) | Mila (voice) |
 Brian DobsonAgent Dunbar / Announcer / Head Chef (voice) | Agent Dunbar / Announcer / Head Chef (voice) |
 Rebecca HusainPatricia (voice) | Patricia (voice) |
 Cathy WeseluckAunt Zoe (voice) | Aunt Zoe (voice) |
 Ian HanlinLazlo (voice) | Lazlo (voice) |
 Jonathan HolmesPercy (voice) | Percy (voice) |
 Kathleen BarrViolet / Alarm (voice) | Violet / Alarm (voice) |