Taking place after the events of Assassin's Creed: Revelations in the year 1524 in Tuscany, follow the retired Assassin Ezio Auditore as he teams up with Shao Jun during his final battles.
| Actor | Role |
|---|---|
 Roger Craig SmithEzio Auditore da Firenze (voice) | Ezio Auditore da Firenze (voice) |
| AAnna TuveriSofia Sartor (voice) | Sofia Sartor (voice) |
 Angela GaluppoFlavia Auditore (voice) | Flavia Auditore (voice) |
 Peter ArpesellaYoung Italian Man (voice) | Young Italian Man (voice) |