The story is the bridges the gap of Assassin's Creed II and Assassin's Creed Brotherhood. After the destruction of Monteriggioni in the year 1500, Ezio met with Leonardo Da Vinci to gather information about the Pope's son, Cesare Borgia. Leonardo tells Ezio of Cesare's rise to power.
| Actor | Role |
|---|---|
 Roger Craig SmithEzio Auditore (voice) | Ezio Auditore (voice) |
 Carlos FerroLeonardo da Vinci (voice) | Leonardo da Vinci (voice) |
 Andreas ApergisCesare Borgia (voice) | Cesare Borgia (voice) |
 Arthur HoldenOctavien de Valois (voice) | Octavien de Valois (voice) |
 Harry StandjofskiJuan Borgia / Oliverotto da Fermo (voice) | Juan Borgia / Oliverotto da Fermo (voice) |