During World War II, a teenage Jewish girl named Anne Frank and her family are forced into hiding in the Nazi-occupied Netherlands.
| Actor | Role |
|---|---|
| RReina TakahashiAnna Frank (voice) | Anna Frank (voice) |
 Gō KatōOtto Frank (voice) | Otto Frank (voice) |
 Fumie KashiyamaEdith Frank (voice) | Edith Frank (voice) |
| SSeiko TanoMargot Frank (voice) | Margot Frank (voice) |
 Jiro SakagamiHermann van Daan (voice) | Hermann van Daan (voice) |
 Tsuyoshi KusanagiPeter van Daan (voice) | Peter van Daan (voice) |
 Yūsuke TakitaAlbert Düsseldorf (voice) | Albert Düsseldorf (voice) |
| YYoshie TairaMiep Gies (voice) | Miep Gies (voice) |
 Tetsuko KuroyanagiPetronella Van Daan (voice) | Petronella Van Daan (voice) |
| TTakurou NakayoshiJohannes Kleiman (voice) | Johannes Kleiman (voice) |
| NNaomi SekidaJopie (voice) | Jopie (voice) |
 Go EndouProfessor Kepler (voice) | Professor Kepler (voice) |
| TToru TakeuchiGreengrocer's owner (voice) | Greengrocer's owner (voice) |
| KKeishi KawaguchiAnnouncer (voice) | Announcer (voice) |
| HHiroyuki KawadaGerman soldier (voice) | German soldier (voice) |
 Riki KagamiGerman soldier (voice) | German soldier (voice) |
| TTetsuo MaekawaMan (voice) | Man (voice) |