Two years after the events of Galaxy Express 999, Earth has become a battlefield, and Tetsuro is summoned to board the Three-Nine once more. In this, the shattering, full-length theatrical conclusion to Leiji Matsumoto's epic story, all questions will be answered and all mysteries will be revealed as Tetsuro embarks on a journey which will reveal a secret so awful, even Maetel herself can hardly bear to speak of it.
| Actor | Role |
|---|---|
 Masako NozawaTetsuro Hoshino (voice) | Tetsuro Hoshino (voice) |
 Masako IkedaMaetel (voice) | Maetel (voice) |
 Reiko TajimaEmeraldas (voice) | Emeraldas (voice) |
 Kaneta KimotsukiThe Conductor (voice) | The Conductor (voice) |
 Tohru EmoriFaust (voice) | Faust (voice) |
 Ryoko KinomiyaPromethium (voice) | Promethium (voice) |
 Kei TomiyamaMeowdar (voice) | Meowdar (voice) |
 Yoko AsagamiMetalmena (voice) | Metalmena (voice) |
 Makio InoueCaptain Harlock (voice) | Captain Harlock (voice) |
 Noriko OharaMime (voice) | Mime (voice) |
 Chiyoko KawashimaKei Yûki (voice) | Kei Yûki (voice) |
 Hidekatsu ShibataLocomotive (voice) | Locomotive (voice) |
 Akiko TsuboiKanae Hoshino (Tetsurô's mother) (voice) | Kanae Hoshino (Tetsurô's mother) (voice) |
 Chikao OhtsukaCaptain Guerilla (voice) | Captain Guerilla (voice) |
 Shūichirō MoriyamaOld Partisan (voice) | Old Partisan (voice) |