A 44-Calibre Mystery is a Silent Western short.
| Actor | Role |
|---|---|
 Harry CareySheriff Cheyenne Harry | Sheriff Cheyenne Harry |
 Claire Du BreyKitty Flanders | Kitty Flanders |
| FFrank MacQuarrieBob Flanders | Bob Flanders |
| VVester PeggPete McGuire | Pete McGuire |
 William SteeleDeputy Sheriff Horton | Deputy Sheriff Horton |
 Hoot GibsonJoe | Joe |
| MMaude EmoryNot listed | Not listed |