 John WayneRobert Marmaduke Sangster Hightower | Robert Marmaduke Sangster Hightower |
 Pedro ArmendárizPedro 'Pete' Roca Fuerte | Pedro 'Pete' Roca Fuerte |
 Harry Carey Jr.William Kearney ('The Abilene Kid') | William Kearney ('The Abilene Kid') |
 Ward BondPerley 'Buck' Sweet | Perley 'Buck' Sweet |
 Mae MarshMrs. Perley Sweet | Mrs. Perley Sweet |
 Mildred NatwickThe Mother | The Mother |
 Jane DarwellMiss Florie | Miss Florie |
 Guy KibbeeJudge | Judge |
 Dorothy FordRuby Latham | Ruby Latham |
 Ben JohnsonPosse man #1 | Posse man #1 |
 Charles HaltonOliver Latham | Oliver Latham |
 Hank WordenDeputy Curly | Deputy Curly |
 Jack PennickLuke | Luke |
 Fred LibbyDeputy | Deputy |
| MMichael DuganPosse Man #2 | Posse Man #2 |
| DDon SummersPosse Man #3 | Posse Man #3 |
 Gertrude AstorSaloon Girl (uncredited) | Saloon Girl (uncredited) |
 Richard HagemanSaloon Pianist (uncredited) | Saloon Pianist (uncredited) |
 Nora BushTownswoman (uncredited) | Townswoman (uncredited) |
 Eva NovakTownswoman (uncredited) | Townswoman (uncredited) |
| CCharles SoldaniTownsman (uncredited) | Townsman (uncredited) |
 Ruth CliffordWoman in Bar (uncredited) | Woman in Bar (uncredited) |
| JJack CurtisBartender (uncredited) | Bartender (uncredited) |
 Harry TenbrookBartender (uncredited) | Bartender (uncredited) |
 Tex DriscollBarfly (uncredited) | Barfly (uncredited) |
 Jack KennyBarfly (uncredited) | Barfly (uncredited) |
 Jack MowerBarfly (uncredited) | Barfly (uncredited) |
 Francis FordDrunk (uncredited) | Drunk (uncredited) |
 Cliff LyonsGuard at Mojave Tanks (uncredited) | Guard at Mojave Tanks (uncredited) |
| AAmelia YeldaRobert William Pedro Hightower (the Baby) (uncredited) | Robert William Pedro Hightower (the Baby) (uncredited) |
